Ion-exchange chromatography (IEX) is one type of separation technique laboratories use. It’s primarily based on the interactions between appropriately charged molecules and stationary phases that have been oppositely charged.
Many laboratories use ion-exchange chromatography to concentrate, purify, and isolate nucleic acids, proteins, and more. While it does take time to prepare samples because they require the right salt and pH concentrations before they’re applied, it is one of the most common ways to test for various illnesses and diseases.
The benefits of ion-exchange chromatography include:
- High Capacity – The resins used in ion-exchange chromatography feature a higher binding capacity. Therefore, it’s easy for labs to purify larger sample amounts in one run. This saves time and money.
- Robustness – IEX is a robust technique used in many labs. It tolerates various salt and pH concentrations, so it’s suitable for various tests.
- Versatile – Ion-exchange chromatography can separate various things, including small and large molecules, nucleic acids, and proteins. Therefore, it’s versatile enough for most laboratory separation needs.
- High Resolution – When labs utilise ion-exchange chromatography, they can achieve a higher resolution and separation efficiency. Therefore, it’s easy to separate closely related compounds without skewing the results of the test.
There are two disadvantages of IEX. The columns used for this separation technique can get fouled over time, so they must be frequently cleaned and replaced. Likewise, pH changes can significantly affect the performance of this method, potentially introducing unwanted contaminants.
One way to avoid the disadvantages and protect the sample is by a haemoglobin diagnostic system that features a high-performance analyser, appropriate software, durable analytical columns, and reagents.
Trinity Biotech has created its Premier Resolution System, which has been used in laboratories around the world. Recently, it received clearance from the FDA (Food and Drug Administration) in the US. Now, it’s easier for labs in the United States to quantify and fractionate haemoglobin variants in adults and children.
Learn more about ion-exchange chromatography and Trinity Biotech today.
