The Synaptophysin antibody helps you recognize proteins with a molecular weight of 38kDa, which is also identified as synaptophysin. It can help you label normal neuroendocrine cells of the adrenal medulla, carotid body, pituitary gland, skin, thyroid, lung, gastrointestinal mucosa, and pancreas of humans. The anti-synaptophysin antibody can react with neuroendocrine neoplasms of neural and epithelial types, including pheo-chromacytomas, ganglioneuromas, ganglioneuroblastomas, neuroblastomas, chromaffin, and more.
It is designed to be used for research purposes and has no available clone at this time. The immunogen is the synthetic peptide of the synaptophysin in humans. The isotype is the Rabbit IgG and has an undetermined epitope. The molecular weight is 38kDa, and it has been tested in humans.
Applications
This antibody is designed to be used with Immunohistochemistry applications. You should prepare the specimen using a Formalin-fixed or paraffin-embedded tissue that is suitable. Likewise, you should use deparaffinized slides that have been cleaned with graded alcohols, xylene, or an alternative to xylene.
When you choose to use the concentrated formula, you will dilute the antibody using a ratio of 1:300. However, you can also find pre-diluted formulations available, making one less step for the lab.
To retrieve the antigen, you will boil the tissue sections using a 10mM citrate buffer with a pH of 6.0 for about 10 minutes, and then allow it to cool to room temperature for 20 minutes. After the cooling period, you should incubate it for 10 minutes at room temperature.
The positive control for the Synaptophysin antibody is the pancreas or the pheochromocytoma with a cellular localization in the cytoplasm.
Synaptophysin can help you label a variety of cells in a variety of areas in the body, including the skin. Visit Spring Bioscience to learn more and find out how to purchase this product for use in your laboratory.
